Table 1 Comparison of EC-network assembly technologies. This table indicates the comparison of the period of EC network formation and connection to host blood circulation after transplantation among three assembly technologies, scaffold, and nanofilm coating and cell sheet technology
From: Introduction of vasculature in engineered three-dimensional tissue
Technology | EC | EC network cultivation periods | Cocultured cells | Ratio of EC | Function as blood vessels checked in vivo | References |
|---|---|---|---|---|---|---|
Scaffold (PLLA-PLGA) | HUVEC | 3~7 days | Fibroblast Skeletal muscle cells | 10~80% | Done (day 10) | |
Nano film coating | HUVEC | 3 day | Fibroblast, MSC, iPS, myocardial cells | 9% | non | [29] |
Cell sheet | Rat EC, HUVEC, | 1〜3 days | Fibroblast, SMC, myocardial cell | 8~10% | Done (within 24 h) |