Fig. 3

Representative intravital two-photon microscopy images of ectopic bone formation area in Col2.3-ECFP mice (visualization of calcium deposition). Visualization of osteoblasts, newly formed bone (collagen), and calcium deposition stained with alizarin after implantation of a collagen sponge containing BMP-2 [15]. Cyan, osteoblasts expressing Col2.3-ECFP; red, calcium deposition stained with alizarin; blue, collagen fibers (second harmonic generation, SHG). Scale bar, 100 μm