Fig. 2

Changes of Iba-1, CD68, CX3CL1, and CX3CR1 expressions within mouse retina induced by ONT and the effects of Anti-CX3CR1 injected intravitreally on retinal microglia and RGCs. A Protein expressions of Iba-1, CD68, CX3CL1, and CX3CR1 in mouse retina at 0, 1, 2, 3, and 4 weeks of ONT (n = 6); B, mRNA expressions of Iba-1, CD68, CX3CL1, and CX3CR1 in mouse retina at 0, 1, 2, 3, and 4 weeks of ONT (n = 6). C Labeling of microglia in ONT retina. Representative images depict the immunohistochemistry for microglia labeled by Iba-1, with white arrows pointing to microglia D Labeling of CX3CL1 in the ONT retina. Representative images depict the immunohistochemistry for CX3CL1 distributed in the retinal ganglion cell layer (GCL). E, Western blot analysis and densitometry quantification of CX3CL1, CD68, and Iba-1 expressions in the sham-operated + PBS group, sham-operated + Anti-CX3CR1 group, ONT 1 week + PBS group, and ONT 1 week + Anti-CX3CR1 group (n = 6). F Western blot analysis and densitometry quantification of βIII-Tubulin, BRN3A, and GAP-43 expressions in the sham-operated + PBS group, sham-operated + Anti-CX3CR1 group, ONT 1 week + PBS group, and ONT 1 week + Anti-CX3CR1 group (n = 6). G Labeling of βIII-Tubulin in ONT retina of in sham-operated + PBS group, sham-operated + Anti-CX3CR1 group (n = 6). Representative images depict the immunohistochemistry for RGCs and their axons labeled by βIII-Tubulin. Magnification 200, scale bar = 50 μm; Bars indicate mean, while error bars show standard deviation. *P < 0.05, **P < .01, ***P < .001, ****P < .0001 versus the control group