Fig. 4

The effects of 48 h delayed intravitreal injection of CX3CL1 on expression of CX3CR1, CD68, and Iba-1. A Western blot analysis and densitometry quantification of CX3CR1, CD68, and Iba-1 expressions within mouse retina in PBS and CX3CL1 delayed injection groups (n = 6). B mRNA expressions of CX3CR1, CD68 and Iba-1 within mouse retina in PBS and CX3CL1 delayed injection groups (n = 6). C Immunofluorescence staining for Iba-1 in PBS and CX3CL1 delayed injection groups. Representative images show the immunohistochemistry for Iba-1 (red), and DAPI (blue), with white arrows pointing to microglia, magnification 200, scale bar = 50 μm. Bars indicate the mean, while error bars show the standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus the PBS injected group