Fig. 5

The effects of 48 h delayed intravitreal injection of CX3CL1 on expressions of βIII-Tubulin, BRN3A, and GAP-43. A Western blot analysis and densitometry quantification of βIII-Tubulin, BRN3A, and GAP-43 expressions within mouse retina in PBS and CX3CL1 delayed injection groups (n = 6). B mRNA expressions of βIII-Tubulin, BRN3A, and GAP-43 within mouse retina in PBS and CX3CL1 delayed injection groups (n = 6). C Immunofluorescence staining and quantitative analysis for RGCs in PBS and CX3CL1 delayed injection groups. Representative images show the immunohistochemistry for RGCs labeled by BRN3A. D Immunofluorescence staining for βIII-Tubulin in PBS and CX3CL1 delayed injection groups. Representative images show the immunohistochemistry for RGCs and axons labeled by βIII-Tubulin; magnification 200, scale bar = 50 μm. E Immunofluorescence staining for GAP43 in CX3CL1 delayed injection group. A swelling (red asterisk) was seen at the tip of the regenerating buds of RGC in ONT mice after intravitreal injection of CX3CL1. F F-VEP detection waveforms of ONT mice in PBS or CX3CL1 injection groups and quantitative analysis of the latency and amplitude of P1 (n = 6). Bars indicate the mean, while error bars show the standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus the PBS injected group