Fig. 5

The OSM-driven macrophage-synovial fibroblast circuit in RA synovial cells. A Main cell clusters (left) and expression of the representative markers (right) by analysis of the integrated scRNA-seq data of RA synovial cells. B Sub-clusters of the integrated RA synovial cells. C Expression of representative markers of RA synovial fibroblast sub-clusters. D Module scores of mouse CIA fibroblasts markers identified 4 mouse CIA fibroblast subpopulations (upper left) as well as CIA fibro_1 2 and 3 (upper right, lower left and lower right, respectively) in RA synovial cells. E Markers of RA synovial myeloid sub-clusters. F Average expression of IL6, OSM, LIF, TNFSF11, TNFRSF11A, MMP13 and JAK/STAT cytokine receptor genes in the RA synovial cell clusters. G Heatmaps of JAK/STAT cytokine-receptor interactions in RA synovial myeloid and fibroblast subsets. H Dot plots of JAK/STAT cytokines-receptors and RANKL/RANK interactions in RA myel_2 and fibro_1-9