Fig. 1

Deletion of TP signaling exacerbates APAP-induced liver injury. A ALT levels after APAP treatment in WT and TP^−/− mice. Data are expressed as the mean ± SD (n = 5–6 mice per group). ****p < 0.0001. B Representative photos of H&E staining of liver sections from WT and TP^−/− mice after APAP treatment. The yellow dotted lines indicate areas of necrosis. CV central vein, PV portal vein. Scale bars: 200 μm. Hepatic necrotic area in WT and TP^−/− mice after APAP treatment. Data are expressed as the mean ± SD (n = 4–6 mice per group). ****p < 0.0001. C mRNA levels of Tp and Txs in the livers from WT and TP^−/− mice after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). D TXB₂ levels in the livers from WT and TP^−/− mice after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). E Double-immunofluorescence staining for TP (green) and CD68 (red) or TXS (green) and CD68 (red). The three right panels are magnified images of the area outlined by the white solid box in the left panel. Scale bar: 20 μm. Arrowheads indicate merged cells. CV central vein