Fig. 3

TP signaling deficiency in bone marrow cells exacerbates APAP-induced liver injury and delays liver repair. A Representative immunofluorescence images of GFP (green) and CD68 (red) in the liver of WT mice bearing GFP⁺ bone marrow (BM) cells 48 h after APAP treatment. The nuclei were stained with DAPI (blue). CV central vein. The three right panels are magnified images of the area outlined by the white solid box in the left panel. Scale bar: 20 μm. Arrowheads indicate merged cells. CV central vein. The percentage of CD68⁺ cells positive for GFP in the livers of WT mice bearing GFP⁺ BM cells 48 h after APAP treatment. Data are expressed as the mean ± SD (n = 5 mice per group). B ALT levels 48 h after APAP treatment in BM chimeric mice. Data are expressed as the mean ± SD (n = 6 mice per group). C Representative images showing H&E staining of liver sections. CV central vein, PV portal vein. Scale bars: 200 μm. Hepatic necrotic areas 48 h after APAP treatment in BM chimeric mice. Data are expressed as the mean ± SD (n = 4–6 mice per group). ***p < 0.001. D Expression of mRNA encoding Il1b and Il6 in the liver 48 h after APAP treatment in BM chimeric mice. Data are expressed as the mean ± SD (n = 5–6 mice per group). *p < 0.05; **p < 0.01; ***p < 0.001. E Representative images showing immunohistochemical staining of PCNA at 48 h after APAP treatment in BM chimeric mice. CV central vein, PV portal vein. Scale bars: 100 μm. Percentage of PCNA⁺hepatocytes in BM chimeric mice. Data are expressed as the mean ± SD (n = 5–6 mice per group). *p < 0.05; **p < 0.01; ***p < 0.001. F Representative images showing immunofluorescence staining for CD68 48 h after APAP treatment in BM chimeric mice. CV central vein, PV portal vein. Scale bars: 100 μm. Number of CD68⁺ macrophages in the BM of chimeric mice. Data are expressed as the mean ± SD (n = 4–5 mice per group). *p < 0.05; **p < 0.01; ***p < 0.001