Fig. 4

Deletion of TP signaling in macrophages delays liver repair after APAP treatment. A ALT levels after APAP treatment in Control and TP^△mac mice. Data are expressed as the mean ± SD (n = 6 mice per group). ***p < 0.001. B Representative photos of H&E staining of liver sections from Control and TP^△mac mice after APAP treatment. The yellow dotted lines indicate areas of necrosis. CV central vein, PV portal vein. Scale bars: 200 μm. Hepatic necrotic area (%) after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). ****p < 0.0001. C Representative images of PCNA immunostaining of liver sections from Control and TP^△mac mice 48 h after APAP treatment. PV portal vein. Scale bars: 100 μm. Percentage of PCNA⁺ hepatocytes 48 h after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). ***p < 0.001. D GSH and GSSG concentrations in the livers of Control and TP^△mac mice after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). E Immunofluorescence of CD68 (red) in the livers of Control and TP^△mac mice 48 h after APAP treatment. CV central vein, PV portal vein. Scale bars: 100 μm. The numbers of CD68⁺ macrophages in livers from Control and TP^△mac mice after APAP treatment. Data are expressed as the mean ± SD (n = 4–6 mice per group). ***p < 0.001. F Expression of genes encoding Ccl2, Ccl7, and Ccl9 in the livers of control and TP^△mac mice 0 h and 48 h after APAP treatment. Data are expressed as the mean ± SD (n = 5–6 mice per group). ***p < 0.001; ****p < 0.0001