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Fig. 11 | Inflammation and Regeneration

Fig. 11

From: Macrophage depletion in inflamed rat knees prevents the activation of synovial mesenchymal stem cells by weakening Nampt and Spp1 signaling

Fig. 11

Proliferative and chondrogenic abilities of synovial MSCs treated with Spp1 and Nampt. A Experimental scheme. Rat synovial MSCs were treated with recombinant human Spp1 and Nampt for 7 days, followed by cell counting and chondrogenic assays. B Relative cell numbers on day 7. Data are presented as the mean ± SD of three replicate wells. C Representative images of cells stained with crystal violet. D Representative images of gross appearance and safranin O staining of chondrogenic pellets. E Pellet wet weight and quantification of glycosaminoglycan (GAG) and DNA. The GAG content was standardized to DNA content (GAG/DNA). Data are presented as the mean ± SD of three replicate pellets. F Quantification of chondrogenic genes. Data are presented as the mean ± SD of three replicate pellets. *p < 0.05

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Inflammation and Regeneration

ISSN: 1880-8190

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