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Fig. 5 | Inflammation and Regeneration

Fig. 5

From: CD157+ vascular endothelial cells derived from human-induced pluripotent stem cells have high angiogenic potential

Fig. 5

Tube formation assay and transplantation using CD31⁺ CD157+ and CD31⁺ CD157− cell populations. a Tube formation assay using endothelial cells on day 11. Red indicates acetylated low density lipoprotein (Alexa Fluor™ 594-Ac-LDL) uptake by endothelial cells. b Tube formation assay using endothelial cells from days 14 and 24. Red indicates Alexa Fluor™ 594-Ac-LDL. scale bar = 500 μm. c Image J Angiogenesis Analyzer results of tube formation assay observed in b. d Quantification, by ImageJ Angiogenesis Analyzer, of nodes, branches, and total length of endothelial cells (**P < 0.01). e Percentage of human CD31+ cells in hind limbs after transplantation of CD31+/CD157+ endothelial cells isolated from a day 14 culture or CD31+/CD157.− endothelial cells isolated from a day 24 culture. n = 3. (**P < 0.01)

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Inflammation and Regeneration

ISSN: 1880-8190

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