Fig. 6

Transplantation of day 14 iPSC-ECs improved lower limb ischemia more effectively than day 24 iPSC-ECs. Representative LDBF images (a) and a line graph of the calculated LDBF ratio (b) are shown. a Blood flow is visualized as changes in laser Doppler frequency using pseudocolor imaging. A significant increase in blood flow was observed in the group transplanted with day 14 iPSC-ECs compared to the day 24 group. b Quantitative analysis of the ischemic/non-ischemic LDBF ratio in mice transplanted with day 14 iPSC-ECs (red) or day 24 iPSC-ECs (blue). n = 4 per group. (*P < 0.05, **P < 0.01)